Assays

ID	Title	Description	Biological Objects Targetted	Biological Processes Targetted
1	Electrocardiogram	This is for test purposes	heart, UBERON:0000948	Abnormal EKG, HP:0003115	View Details
2	Lung Histopathology		lung ciliated cell, CL:1000271	inflammatory cell apoptotic process, GO:0006925	View Details
3	Flow Cytometry for Treg Cells		regulatory T cell, CL:0000815	Immunosuppression, D007165	View Details
4	Western blot analysis	Detects protein extracted from cell, tissue, organ or surrounding liquid (e.g. interstitium, tis...	angiotensin-converting enzyme 2, PR:000003622	membrane protein ectodomain proteolysis, GO:0006509	View Details
5	Detection of ACE2 protein level	Monoclonal antibodies against the ACE2 ectodomain (R&D Systems) - doi:10.1073/pnas.0711241105 Ra...	angiotensin-converting enzyme 2 (human), PR:Q9BYF1	membrane protein ectodomain proteolysis, GO:0006509	View Details
6	ARE-Nrf2 Luciferase Test Method for evaluation of Keratinocyte activation	Luc reporter gene expression assay in HaCaT human keratinocytes stably transfected with luciferas...	nuclear factor erythroid 2-related factor 2, PR:000011170	keratinocyte activation, GO:0032980	View Details
7	Aromatase Inhibition	Detection of aromatase inhibition in fathead minnow ovary tissue by a selection of chemicals know...	Ovary, FMA:7209	response to aromatase inhibitor, GO:0061477	View Details
8	TEST		heart valve cell, CL:1000147		View Details
9	RTgill-W1 cell line assay (fish cell line acute toxicity, OECD TG 249)	Fish cells (permanent cell line from rainbow trout gill, RTgill-W1), cultured in a 24-well plate ...	cell, GO:0005623	cell death, GO:0008219	View Details
10	Fish Embryo Acute Toxicity (FET) Test with the zebrafish	Eggs of zebrafish (Danio rerio) are exposed to the test chemical from birth (newly fertilized) un...	embryo, UBERON:0000922	embryonic lethality, MP:0008762	View Details
11	EASZY assay: Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos	This is a "mechanism-based in vivo screening assay designed to detect endocrine active chemicals ...	xenoestrogen, CHEBI:76988	estrogen receptor activity, GO:0030284	View Details
12	NVS_ADME_hCYP19A1 (ToxCast Assay Endpoint Aeid 319)	In chemico (cell free) assay employed to measure aromatase (CYP19) enzyme activity. The assay use...	aromatase, PR:000006100	aromatase activity, GO:0070330	View Details
13	TOX21_Aromatase_Inhibition (ToxCast assay endpoint Aeid 767)	TOX21_Aromatase_Inhibition is one of one assay component(s) measured or calculated from the TOX21...	aromatase, PR:000006100	aromatase activity, GO:0070330	View Details
14	Aromatase activity by tritiated water release.	Tritiated androstenedione, labeled in the 1beta position, is incubated with NADPH in the presence...	aromatase, PR:000006100	aromatase activity, GO:0070330	View Details
15	Sex Ratio	Sex ratio is the phenotypic proportion of the categories female, male, intersex, and undifferenti...	gonad, UBERON:0000991	sex determination, GO:0007530	View Details
16	Vitellogenin	Induction of vitellogenin (VTG) is a biomarker for exposure to estrogens and aromatase inhibitors...	vitellogenins, D014819	sex determination, GO:0007530	View Details
17	Organochlorine pesticide detection		Blood cell, FMA:62844		View Details
18	Skin Sensitisation: the human Cell Line Activation Test (h-CLAT)	The human Cell Line Activation Test (h-CLAT) is used to contribute to the assessment of the skin ...			View Details
19	Voltage clamp recording	Voltage clamp recordings currents evoked by choline and acetylcholine (ACh) obtained in cExNPCs, ...	electrically responsive cell, CL:0000393	neuronal action potential, GO:0019228	View Details
20	Transthyretin (TTR) Binding Assay	ANSA (8-anilino-1-naphthalenesulfonic acid ammonium salt) fluorescence displacement assay, compet...	transthyretin, PR:000016801	regulation of protein binding, GO:0043393	View Details
21	Fish Short Term Reproduction Assay	This Test Guideline describes an in vivo screening assay for fish reproduction where sexually mat...	adult organism, UBERON:0007023	sexual reproduction, GO:0019953	View Details
22	Inflammatory cellular response	HepG2 hepatocyte cells were measured for concentrations of pro-inflammatory cytokines after being...	hepatocyte, CL:0000182	inflammatory cell apoptotic process, GO:0006925	View Details
23	Measurement of plasma vitellogenin in fathead minnows by competitive ELISA	This procedure is used at the US EPA Mid-Continent Ecology Division (now Great Lakes Toxicology a...			View Details
24	Test Assay				View Details