Details for Assay 10: Fish Embryo Acute Toxicity (FET) Test with the zebrafish
Description
Eggs of zebrafish (Danio rerio) are exposed to the test chemical from birth (newly fertilized) until 96 hours post fertilization. Developing embryos are observed every 24 hrs to record specific apical observations indicative of lethality according to the OECD TG 236. In addition, a number of apical observations indicative of sub-lethal effects can be made (not part of the OECD TG, but widely used), and further assays (e.g., behavioral, biochemical, omics analyses) can be performed as well using embryo samples generated in this assay.Validation Notes
The test based on four apical observations indicative of lethality was validated in a round-robin prior to its acceptance as the OECD TG 236.No biological action information submitted
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
| Test No. 236: Fish Embryo Acute Toxicity (FET) Test | OECD | 2013 | https://doi.org/10.1787/9789264203709-en |
Technical Details
| Detection Technology | Biological Objects Targetted | Biological Processes Targetted |
|---|---|---|
| Primary: microscopical observations. Four apical observations indicative of lethality (according to the OECD TG236) include (i) coagulation of fertilized eggs, (ii) lack of somite formation, (iii) lack of detachment of the tail-bud from the yolk sac, and (iv) lack of heartbeat. Sub-lethal effects can also be detected based on microscopical observations (e.g. jaw or axis deformities) or using further assays, e.g. biochemical, omics or behavioral analyses. |
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