Details for Assay 20: Transthyretin (TTR) Binding Assay
Description
ANSA (8-anilino-1-naphthalenesulfonic acid ammonium salt) fluorescence displacement assay, competitive TTR binding measured by decrease in relative fluorescence in well.Validation Notes
2.5% maximal solvent concentration for ANSA-TTR experiments. Assay should be conducted in black, round-bottom 96-well plates, with fluorescence measured at well bottom.No biological action information submitted
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
| New Approaches to Assess the Transthyretin Binding Capacity of Bioactivated Thyroid Hormone Disruptors | Mauricio Montaño, Emmanuelle Cocco, Cedric Guignard, Göran Marsh, Lucien Hoffmann, Åke Bergman, Arno Christian Gutleb, Albertinka Jacoba Murk | 2012 | https://doi.org/10.1093/toxsci/kfs228 |
Technical Details
| Detection Technology | Biological Objects Targetted | Biological Processes Targetted |
|---|---|---|
| Fluorescent Microplate Reader |
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