Details for Assay 6: ARE-Nrf2 Luciferase Test Method for evaluation of Keratinocyte activation

Description

Luc reporter gene expression assay in HaCaT human keratinocytes stably transfected with luciferase gene under the transcriptional control of the SV40 promoter fused with the ARE from the AKR1C2 gene.

Validation Notes

Validated for assessment of keratinocyte activation (KE826) leading to Skin Sensitisation (AOP40). It may be useful to be able to put a link the ECVAM site that describes the steps of validation and related docs

No biological action information submitted

Reference Details

Title	Authors	Year	DOI
ARE-Nrf2 Luciferase Test Method for Keratinocyte activation	OECD

Technical Details

Detection Technology	Biological Objects Targetted	Biological Processes Targetted
Luminescence detection of luciferase activity (using well established substrates) as a proxy for the Luc gene induction/activation by the transcription factor Nrf2 known to increase in cells following exposure to electrophilic test substances (ref). Latter are associated with a potential for skin sensitisatio (ref) but also other types of toxicity (e.g. oxidative stress - ref).	nuclear factor erythroid 2-related factor 2, PR:000011170	keratinocyte activation, GO:0032980

Go to Assays Table