Details for Assay 5: Detection of ACE2 protein level

Description
Monoclonal antibodies against the ACE2 ectodomain (R&D Systems) - doi:10.1073/pnas.0711241105 Rabbit polyclonal antibodies against the ACE2 cytoplasmic domain (MBL Corp) - doi:10.1073/pnas.0711241105 Goat anti-ACE2 primary polyclonal antibody (No. AF933; R&D Systems, Minneapolis, MN) - in DOI 10.1152/ajplung.00071.2009
Validation Notes
Validation in terms of what? The reference shows that the Abs used detect full length and specific deletion mutants expressed from a vector encoding human ACE2 (or mutants)

No biological action information submitted

Reference Details
Title Authors Year DOI
Modulation of TNF-alpha-converting enzyme by the spike protein of SARS-CoV and ACE2 induces TNF-alpha production and facilitates viral entry Shiori Haga, Norio Yamamoto, Chikako Nakai-Murakami, Yoshiaki Osawa, Kenzo Tokunaga, Tetsutaro Sata, Naoki Yamamoto, Takehiko Sasazuki, Yukihito Ishizaka 2008 https://doi.org/10.1073/pnas.0711241105
Technical Details
Detection Technology Biological Objects Targetted Biological Processes Targetted
Western blotting and anti ACE2 antibodies Visualizing the ACE2-primary body complex: in doi:10.1073/pnas.0711241105 not specified; in doi: 10.1152/ajplung.00071.2009 HRPconjugated mouse anti-goat secondary A
  • angiotensin-converting enzyme 2 (human), PR:Q9BYF1
  • membrane protein ectodomain proteolysis, GO:0006509
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