Details for Assay 16: Vitellogenin
Description
Induction of vitellogenin (VTG) is a biomarker for exposure to estrogens and aromatase inhibitors. VTG can be quantified in tissue homogenate or plasma by ELISA.Validation notes not yet submitted
No biological action information submitted
Key Events Referencing this Assay
| Key Event ID | Title | Short Name |
|---|---|---|
| 221 | Reduction, Plasma vitellogenin concentrations | Reduction, Plasma vitellogenin concentrations |
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
| Comparison of zebrafish (Danio rerio) and fathead minnow (Pimephales promelas) as test species in the Fish Sexual Development Test (FSDT) | Holbech et al. | 2012 | https://doi.org/10.1016/j.cbpc.2011.11.002 |
Technical Details
| Detection Technology | Biological Objects Targetted | Biological Processes Targetted |
|---|---|---|
| ELISA (direct, non-competitive, sandwich: plates coated with anti-lipovitellin IgG, biotin coupled to the IgG, and poly-HRP streptavidin and TMB plus added, plates read at 450nm) |
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