Details for Assay 12: NVS_ADME_hCYP19A1 (ToxCast Assay Endpoint Aeid 319)
Description
In chemico (cell free) assay employed to measure aromatase (CYP19) enzyme activity. The assay uses human CYP19A1 protein. Changes to fluorescence intensity signals produced from an enzymatic reaction [Reaction:DBOMF+ NADPH -> fluorescein + NADP+] involving the key substrate [Di(benzyloxymethoxy)fluorescein (DBOMF)] are indicative of changes in enzyme function and kinetics for the human cytochrome P450, family 19, subfamily A, polypeptide 1 [GeneSymbol:CYP19A1 | GeneID:1588 | Uniprot_SwissProt_Accession:P11511].Validation Notes
https://comptox.epa.gov/dashboard/assay-endpoints/NVS_ADME_hCYP19A1No biological action information submitted
Key Events Referencing this Assay
| Key Event ID | Title | Short Name |
|---|---|---|
| 36 | Inhibition, Aromatase | Inhibition, Aromatase |
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
Technical Details
| Detection Technology | Biological Objects Targetted | Biological Processes Targetted |
|---|---|---|
| Fluorescence |
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