Details for Assay 11: EASZY assay: Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos
Description
This is a "mechanism-based in vivo screening assay designed to detect endocrine active chemicals acting as agonist through estrogen receptors (ERs), by inducing the expression of the green fluorescent protein (GFP) driven by the cyp19a1b promoter".Validation Notes
Adopted as OECD Test Guideline No. 250No biological action information submitted
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
| Test No. 250: EASZY assay - Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos | OECD | 2021 | https://doi.org/10.1787/0a39b48b-en |
Technical Details
| Detection Technology | Biological Objects Targetted | Biological Processes Targetted |
|---|---|---|
| Transgenic tg(cyp19a1b:GFP) Zebrafish embryos are exposed for 96 hours during embryonic development and GFP fluorescence in the brain is compared between control and exposed subjects by fluorescent microscopy and image analysis. |
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