Details for Assay 7: Aromatase Inhibition

Description
Detection of aromatase inhibition in fathead minnow ovary tissue by a selection of chemicals known to be aromatase inhibitors in human aromatase protein (NVS_ADME_hCYP19A1) and human cell line (Tox21_Aromatase_Inhibition).
Validation Notes
Test

No biological action information submitted

Reference Details
Title Authors Year DOI
Case Study in 21st Century Ecotoxicology: Using In Vitro Aromatase Inhibition Data to Predict Short‐Term In Vivo Responses in Adult Female Fish Villeneuve, Daniel L., Brett R. Blackwell, Jenna E. Cavallin, Wan‐Yun Cheng, David J. Feifarek, Kathleen M. Jensen, Michael W. Kahl, Rebecca Y. Milsk, Shane T. Poole, Eric C. Randolph, Travis W. Saari, and Gerald T. Ankley 2021 https://doi.org/10.1002/etc.4968
Technical Details
Detection Technology Biological Objects Targetted Biological Processes Targetted
Post-mitochondrial supernatants of ovary tissue incubated with aromatase inhibitor and phosphate buffer containing androstenedione. Aromatase activity determined from tritiated water released during conversion of labeled androstenedione to estrone.
  • Ovary, FMA:7209
  • response to aromatase inhibitor, GO:0061477
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