Details for Assay 13: TOX21_Aromatase_Inhibition (ToxCast assay endpoint Aeid 767)
Description
TOX21_Aromatase_Inhibition is one of one assay component(s) measured or calculated from the TOX21_Aromatase_Inhibition assay. It is designed to make measurements of luciferase induction, a form of inducible reporter, as detected with bioluminescence signals by CellTiter-Glo Luciferase-coupled ATP quantitation technology. Changes to bioluminescence signals produced from an enzymatic reaction involving the key substrate [One-Glo] are indicative of changes in transcriptional gene expression due to modulation of the human cytochrome P450, family 19, subfamily A, polypeptide 1 [GeneSymbol:CYP19A1 | GeneID:1588 | Uniprot_SwissProt_Accession:P11511]. TOX21_Aromatase_Inhibition is a cell-based, single-readout assay that uses MCF-7, a human breast cell line, with measurements taken at 24 hours after chemical dosing in a 1536-well plate.Validation Notes
https://comptox.epa.gov/dashboard/assay-endpoints/TOX21_Aromatase_InhibitionNo biological action information submitted
Key Events Referencing this Assay
| Key Event ID | Title | Short Name |
|---|---|---|
| 36 | Inhibition, Aromatase | Inhibition, Aromatase |
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
Technical Details
| Detection Technology | Biological Objects Targetted | Biological Processes Targetted |
|---|---|---|
| CellTiter-Glo Luciferase-coupled ATP quantitation; Bioluminescence; Luminescence |
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