Details for Assay 23: Measurement of plasma vitellogenin in fathead minnows by competitive ELISA
Description
This procedure is used at the US EPA Mid-Continent Ecology Division (now Great Lakes Toxicology and Ecology Division) to determine the concentration of vitellogenin (Vtg) in plasma of fathead minnows (Pimephales promelas).Validation Notes
US EPA validated; EPA/600/R-01/067No biological action information submitted
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
| A short term test method for assessing the reproductive toxicity of endocrine-disrupting chemicals using the fathead minnow (Pimephales promelas) | US EPA | 2002 | https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=30002HB8.PDF |
Technical Details
| Detection Technology | Biological Objects Targetted | Biological Processes Targetted |
|---|---|---|
| The assay is performed in 96-well ELISA plates that have been coated with purified fathead minnow Vtg. After blocking the unbound sites with goat serum, the plates is washed to removed any unbound material. The sampled being assays, a range of standards prepared with purified fathead minnow vitellogenin, and the appropriate blanks and controls are incubated with an antibody specific to fathead minnow Vtg. |