Study Design details for SD# 3
Reference Details
| Title | Authors | Year | DOI |
|---|---|---|---|
| Modulation of TNF-alpha-converting enzyme by the spike protein of SARS-CoV and ACE2 induces TNF-alpha production and facilitates viral entry | Shiori Haga, Norio Yamamoto, Chikako Nakai-Murakami, Yoshiaki Osawa, Kenzo Tokunaga, Tetsutaro Sata, Naoki Yamamoto, Takehiko Sasazuki, Yukihito Ishizaka | 2008 | https://doi.org/10.1073/pnas.0711241105 |
Study Type: in vitro
Regimen description
not sure about this needs some guidance.
Is it maybe : such and such cells expressing endogenous or tranfected X protein were exposed to/treated with (stressor/regimen e.g. light). The level of X protein ectodomain was measured in the whole cell, cell membrane, and in the medium .
Subject Properties
| Taxon Term | Strain | Sex | Life Stage |
|---|---|---|---|
| Monkey | cell line - Vero E6 kidney epithelial endogenous ACE2 |
Observations
| ID | Stressor | Sample (short_name) | Assay | Effect | Associated Key Events | |
|---|---|---|---|---|---|---|
| 3 | Western blot analysis | decreased |
|